Como citar APA:
Monterrosa-Molina, P., et al. (2024) Analysis of veterinary health
situations in domestic animals. Repique, 6(2), 59-73
Vol. 6 Núm. 2
Julio - Diciembre 2024
e-ISSN: 2550-6676
pp 59-73
Analysis of veterinary health situations in domestic animals
Chacón-Morales Mariella
*
Piña-Paucar Ana
*
Maridueña-Zavala María Isabel
*
Abstract
Crystalluria is a disease that is currently present in more than 50% of
the cat and dog population, causing urinary elimination dysfunction
and therefore clinical signs related to toxin retention. The following
study evaluated the presence of crystalluria in dogs and cats without
clinical signs, attended in two veterinary clinics in Ecuador. Urine
samples were collected from 150 animals, ranging from 80 dogs and
70 cats, of different ages, sexes and body index (BMI). The samples
were extracted by means of the cystocentesis technique, after which
the macroscopic and microscopic characteristics of the urine
collected were identified by means of the uroanalysis technique. The
results obtained allowed us to conclude that more than 55% of the
dogs and cats present crystals, the most frequent types of crystals
were amorphous urate and struvite. It is important to keep in mind
that periodic evaluations allow us to reach a diagnosis in pathologies
that affect the urinary system in order to improve the quality of life of
our patients.
Key words:
Crystalluria, urine, cystocentesis, uroanalysis.
* Mgs. Universidad Agraria del Ecuador, mchacon@uagraria.edu.ec, https://orcid.org/0009-0003-
1111-0029
* Mgs. Universidad Agraria del Ecuador, apina@uagraria.edu.ec, https://orcid.org/0000-0002-1306-
9862
* Mgs. Universidad Agraria del Ecuador, mmariduena@uagraria.edu.ec, https://orcid.org/0000-0002-
8133-1949
Chacón-Morales Mariella, Piña-Paucar Ana, Maridueña-Zavala María Isabel
60
Análsis de situaciones de salud veterinaria en animales
domesticos
Resumen
La cristaluria es una enfermedad que se presenta actualmente en
más del 50% de la población de gatos y perros, causando disfunción
al eliminar la orina y por lo tanto signos clínicos relacionados con la
retención de toxinas. El siguiente estudio evaluó la presencia de
cristaluria en perros y gatos sin signos clínicos, atendidos en dos
clínicas veterinarias, en Ecuador. Se tomaron muestras de orina a 150
animales, comprendidos entre 80 perros y 70 gatos, de diferentes
edades, sexos e índice corporal (IC). Las muestras fueron extraídas
por medio de la técnica de cistocentesis, luego de esto y mediante
la técnica del uroanálisis se identificó las características
macroscópicas y microscópicas de la orina recolectada. Los
resultados obtenidos nos permitieron concluir que más del 55% de
los perros y gatos presentan cristales, los tipos de cristales más
frecuentes fueron los uratos amorfos y la estruvita. Es importante
tener en cuenta que las evaluaciones periódicas nos permiten llegar
al diagnóstico en patologías que afectan al aparato urinario para
mejorar la calidad de vida de nuestros pacientes.
Palabras clave:
Cristaluria, orina, cistocentesis, uroanalisis.
Received : 19-04-2024
Approved: 08-05-2024
INTRODUCTION
The urinary system has important physiological functions such as
the excretion of waste substances, as well as the regulation of water
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61
balance (Marroquín Salcedo & Granados Férnandez, 2021).
Crystals in urine have a relationship with poor nutrition, metabolic
problems or urinary diseases, these can be of acute or chronic
presentation, usually they are recurrent very little known affecting
different species of animals (Lulich et al., 2016) . Crystalluria is the
union of several anatomical, behavioral and nutritional factors,
which can sometimes be identified and corrected in time (Ayala-
Cruz D et al., 2019). The most important factor behind the
formation of uroliths is the state of urinary saturation (Hsu et al.,
2022), there are 3 states of saturation: undersaturated, saturated
and supersaturated and this can be influenced by the magnitude of
renal excretion of crystalloid, urinary pH and/or factors that inhibit
the formation or aggregation of the crystal (Jordán Delgado, 2017).
The diagnosis of crystalluria has increased over the years having as
more evident clinical signs the presentation of irritation in the area
that generates inflammation and discomfort, generally dysuria,
hematuria, stranguria, lethargy, abdominal distension and post-
renal azotemia are presented with higher incidence (Piyarungsri et
al., 2020).
In the case of dogs it not only affects the bladder, but also the
urethra and prostate with clinical signs such as stranguria,
pollakiuria, dysuria and hematuria (Rosas Martínez & García Zárate,
2017), commonly observed in daily clinic. Male dogs are the
patients who most commonly present with these signs due to their
anatomical characteristics, among the most common breeds are
from Labrador, German Shepherd to Cocker Spaniel and
Chihuahua (Ruiz Mendoza, 2021). As for the age in animals that are
reported positive cases of crystalluria, they are from three months
to 8 years old, coming to trigger subsequent problems such as
bacterial urinary tract infections, urination abnormalities,
urolithiasis, prostate diseases, and problems due to trauma
(Mendoza López, 2015). In the literature it has been reported that
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62
in American cities and in Mexico 80% of crystals found in canine
sediments are of the struvite type, in the United States we find that
18% suffer from diseases of the caudal urinary tract, while in Mexico
the percentage is 20% (Aké et al., 2021).
In the case of cats, crystalluria and urolithiasis is responsible for 10
to 23% of the cases of lower urinary tract diseases known as FLUTD
(Feline Low Urinary Tract Disease) (Tefft et al., 2021), presenting in
children under 10 years of age and approximately 10% of cats older
than 10 years (Castaño Jimenez & Londoño Espinosa, 2017),
similarly nephroliths can cause from obstructions at the level of the
ureteropelvic junction, traveling through the ureter until reaching
the kidney (Hsu et al., 2022). The formation of feline uroliths is
multifactorial and depends on diet, metabolism, genetics and
infectious diseases (Hsu et al., 2022).
The present research work aimed to conduct a study to establish
the number of dogs and cats suffering from crystalluria using urine
samples, where the presence of crystals in the urinary sediment of
dogs and cats was determined by uroanalysis, to ensure an accurate
and rapid diagnosis to identify an ideal treatment.
METHODOLOGY
Urine samples from 70 cats and 80 dogs attending veterinary
medical centers in Ecuador were evaluated. The selection of the
patients was randomized and the medical records were filled in with
the information provided by the guardians.
Cystocentesis and Uroanalysis
For urine extraction by cystocentesis, the recommendations of
(Aguiñaga Negrete et al., 2019) were followed where the patient
was immobilized on top of the examination table, then the hair in
the area was shaved and antisepsis was performed (Figure 1). Once
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the area from which the urine sample would be extracted was
prepared, the feline or canine was placed in the dorsal decubitus
position, then the transducer was placed to corroborate the
location in the proper organ (bladder) and the ventral puncture was
performed using a 10 ml syringe. Afterwards, the needle was
inserted at 45º in a caudal cranial direction through the ventral wall
to avoid trauma to the ureters and abdominal vessels and placed in
a test tube.
Figure 1-A.
Echo-guided cystocentesis. 1-B Preparation of the
sample for centrifugation.
The uroanalysis technique allows us to observe blood cells,
epithelial cells, casts and crystals, which is what we wanted to
determine in this study and relate it to the information obtained
from the anamnesis (Abdelgabar et al., 2020). To perform the
uroanalysis, the steps detailed by (Meneses et al., 2021) were
followed, where the macroscopic characteristics of the urine were
first evaluated, such as volume, transparency or turbidity, color and
odor. In the next step, the collected urine was centrifuged at
1500rpm for 5 minutes, the supernatant was removed from the
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64
centrifuged sample and 50ul of the concentrate that remained at
the bottom of the tube was collected with a Pasteur pipette and
placed on a slide. Before observation under the microscope (Zeus)
the sample was covered with coverslips to finally be observed with
a 10X and then 40 X lens.
Statistical design
The approach of this research was quantitative since urine samples
from each of the animals analyzed in the laboratory were analyzed,
as well as a descriptive approach to detail the types of crystals
found in the analyzed urine samples. The data obtained from the
laboratory tests were tabulated and analyzed by means of a
spreadsheet, the data were represented by means of descriptive
tables and graphs. Descriptive statistics were used (Venables &
Smith, 2024).
RESULTS
Population of cats and dogs with the presence of crystalluria.
Figure 2 shows that, of the 80 samples analyzed in dogs, 44 positive
cases were found, equivalent to 55% presence of crystalluria in
dogs and 45% of cases without crystalluria. For the results of
crystalluria in cats, 34 positive cases were detected, representing
48.57%, and 51.43% did not contain crystalluria.
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Figure 2.
Presence of crystalluria in urine sediments of dogs and
cats evaluated in veterinary clinics in Ecuador.
Types of crystals by species
In the results of the types of crystals we can observe that, in the
population of 44 positive dogs, 36% of amorphous urates and in
less quantity struvite crystals with 1% (Figure 3).
Figure 3.
Sediment samples analyzed under the microscope at 40X
zoom in cats or dogs. A) Amorphous urate B) Amorphous urate C
Struvite
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In the case of cats we found that of the 34 that tested positive for
crystals, 64.70% were of the struvite type and 6% amorphous urate
(Figure 4).
Figure 4.-
Bar graph showing the levels of crystalluria found in cats
and dogs of the 150 samples evaluated.
Influence of age, sex, diet and CHF in patients with crystalluria.
To identify whether characteristics such as animal age, sex, type of
diet and body index (BMI) influence the presence of crystalluria in
patients, we compared the results obtained with the information
provided from the medical records. The results of the presence of
crystalluria according to the age of the animal are shown in Table 1
where it is observed that 66% of senior dogs have a higher
percentage of crystalluria, compared to cats that show 64.70% of
crystalluria, but in adulthood.
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Table 1.
Presence of crystalluria according to age.
Regarding the sex of the animal in Table 2 we observe that in dogs,
females are the ones with the highest percentage of crystalluria with
54.54% as opposed to cats that obtained 76.47% of crystalluria.
Table 2.
Presence of crystalluria according to the sex of the animal.
Regarding the type of food consumed by the patients in the study,
Table 3 shows that the two species reached high percentages of
75% in dogs and 85.29% in cats in the consumption of dry balanced
food.
Crystalluria according to age
Dogs
Cats
Number
Percentage
Number
Percentage
Puppy
0
0%
0
0%
Adult
23
52.27%
22
64.70%
Senior
21
66%
12
35.29%
Total
44
100%
34
100%
Crystalluria by sex
Dogs
Dogs
Percentage
Percentage
Female
54,54%
23,52%
Male
45,45%
76,47%
Total
100%
100%
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Table 3.
Presence of crystalluria according to type of food.
Finally, according to the CCI, Table 4 shows that level 3 in dogs has
47.72% of patients with crystalluria, while in cats the highest CCI
levels with presence of crystalluria are 3 and 4 with 38.23%.
Table 4.
Presence of crystalluria according to CI,
Power supply type
dogs
cats
Positive
Porcentage
Positive
Balanced
33
75%
29
Homemade food
11
25%
5
Total
44
100%
34
Body Condition (ICC)
Dogs
Cats
Number
Porcentage
Number
Porcentage
1
0
0%
0
0%
2
4
9,09%
8
23,52%
3
21
47,72%
13
38,23%
4
18
40,90%
13
38,23%
5
1
2,27%
0
0%
Total
44
100%
34
100%
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In the present study 150 animals were evaluated, 80 dogs and 70
cats with presence of crystalluria, in which in general we observed
that more than 50% of cats and dogs showed presence of
crystalluria, similar evaluations in Mexico in 2019 show high levels
of crystalluria in animals attended in consultation (Ayala-Cruz D et
al., 2019) In our study the type of crystalluria that was found in most
of the dogs evaluated was amorphous urates and in smaller
amounts struvite, which differs with other studies conducted in
Mexico in which more than 70% of the samples were struvite (Aké
et al., 2021), and another study conducted in the city of Guayaquil
in cats that showed a similar percentage in the presence of
struvite.(Lozano Pazmiño, 2020).
If we compare factors such as sex, coincidentally we found that in
our samples the most predominant with presence of crystalluria in
dogs are females, similar results have been obtained with urine
samples in dogs in the city of Quito that found more positive cases
in females (Rosero Gómez, 2014), in the case of cats we found a
greater presence of crystalluria in males, similarly when comparing
our results with other studies we found that most of the cases
reported in Venezuela were in males with 63.58% (Polat et al.,
2022). However, the presence of crystals is not a factor linked to
sex, since we have been able to observe that they are present in
both sexes indistinctly, but the anatomical characteristics of males
make them more susceptible to show them.
The feeding by means of dry type balanced feed had a higher
frequency of positivity of crystalluria, compared to other types of
feed, such as homemade, this result is closely related to a study
done in the city of Quito where commercial diets were tested
showing that the percentages of higher presence of crystals were in
animals that consumed this type of feed (Idrovo Torres & Parra
Pacheco, 2020). It should be taken into account that, although this
type of food is the most frequently recommended, because it is
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practical and provides a better supply of nutrients, the results show
that it can generate a greater presence of crystalluria in
symptomatic and asymptomatic patients, so further research is
recommended to evaluate diets and possible sensitivities to this
type of components.
Both in adult dogs and cats a greater presence of crystalluria was
found, similar works reflect that crystalluria is present in all ages
(Aké et al., 2021), however, physiologically it is more common in
adult patients and seniors due to a normal degeneration of the
urinary system organs preventing a normal elimination of the
mineral concentrations coming from the different types of food. In
reference to the body condition index (BCI), dogs presented level
3 and cats level 3 and 4, which shows that most of the animals
evaluated maintain an ideal weight (level 3) and overweight (level
4). It is necessary to take into account that the ICC is related to the
type of food consumed by the animal (Lulich et al., 2016), which
shows us that some of the animals evaluated maintain an
uncontrolled habitual diet which can affect their health and can
favor the formation of mineral concentrations in the urine, presence
of bacteria, or impede the proper functioning of the urinary system.
Therefore, it would be advisable to improve the feeding, which
would allow to reduce the formation of crystals and their
progressive elimination.
CONCLUSIONS
In this study we were able to conclude that more than half of the
animals evaluated had crystalluria, being able to say that it is a
pathology that can be both in symptomatic or asymptomatic
animals, therefore, the performance of timely and rapid
examinations such as a uroanalysis will give us the opportunity to
prevent the appearance of other signs or complications. Sex, age
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and ICC are factors that do not seem to have relevance in the
appearance of crystalluria, however, it is recommended to perform
timely examinations and maintain a quality diet, constant water
consumption in order to avoid factors that predispose to the
appearance of this pathology, especially in animals that have a
genetic predisposition which would alter their health and quality of
life.
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